Methylglyoxal-induced modifications of significant honeybee proteinous components in manuka honey: Possible therapeutic implications

Methylglyoxal (MGO) is a major antibacterial component of manuka honey. Another antibacterial component found in Revamil honey, peptide defensin1, was not identified in manuka honey. The primary aim of the study was to evaluate the content of defensin1 in honeys of different botanical origins and to investigate a presumed effect of reactive MGO on defensin1 and a dominant protein of honey MRJP1 in manuka honey. Immunoblotting of honey samples showed that defensin1 was a regular but quantitatively variable component of honeys. One of the reasons of varying contents of defensin1 in different honeys seems to be constitutive but varying defensin1 expression in individual honeybees in bee populations that we documented on samples of nurse and forager bees by RT-PCR. Comparative analyses of honeys revealed a size modification of defensin1, MRJP1 and probably also α-glucosidase in manuka honey. We further showed that (i) the treatment of purified defensin1 in solution containing high amount of MGO caused a time-dependent loss of its antibacterial activity and (ii) increasing MGO concentrations in a non-manuka honey were connected with a gradual increase in the molecular weight of MRJP1. Obtained results demonstrate that MGO abrogates the antibacterial activity of defensin1 and modifies MRJP1 in manuka honey. We assume that MGO could also have negative effects on the structure and function of other proteins/peptides in manuka honey, including glucose oxidase, generating hydrogen peroxide.     

Production trends and technical efficiencies of culture-based fisheries in five tropical irrigation reservoirs: A case study from Sri Lanka

Culture-based fisheries (CBF) are increasingly accepted as strategies for enhancing inland fisheries, especially in tropical Asia. In Sri Lanka, CBF development in irrigation reservoirs has gained momentum due to concerted efforts of government fisheries authorities for inland fisheries enhancement. In the present study, production trends of five irrigation reservoirs of Sri Lanka before and after the introduction of CBF were investigated and apparently optimal CBF yields were not realised. Hence, the stochastic frontier production function (SFPF) was employed to quantify technical efficiencies (TE) of CBF. For each reservoir, annual averages of input data from 2005 to 2018 (14 years) were used in the TE analysis. Hence, total sample size for estimation of SFPF was 70. Although CBF production gradually increased in all five reservoirs from 2005 to 2018, there were substantial variations of total fish production across the reservoirs. The SFPF indicated that CBF production could be further increased through more efficient management of inputs (i.e. number of fishers, mean number of fishing days per year and stocking density).     

对高职会计专业学生实践能力培养的思考

会计专业人才实践能力的培养是一个循序渐进的过程，在教学过程中，我们采取分阶段、分步骤的实践教学策略，基本实现了高职会计专业人才实践能力的培养目标。     

日语作为英语专业第二外语的思考

从中文和日文,英文和日文的相同点和差异上阐述了日语是适合英语专业学生学习的一门第二外语。     

紫薇洛瘤蛾的生物学特性

研究了紫薇洛瘤蛾Ｍｅｇａｎｏｌａ ｍａｊｏｒ在江西南昌一年发生代数，生活习性，各虫态历期，并提出了防治措施。     

畜禽数量性状主效基因及基因集团的检测

本文详述了离数量性状主效基因和基因集团的检测,定位及效率估计的发展现状,并对各种检测方法进行了评价。     

非英语专业学生不同任务下阅读策略研究

非英语专业大学英语阅读大概可以分为三种任务类型: 课堂阅读、课外阅读和考试阅读。本研究主要以 O''Malley & Chamot 的学习策略分类框架为基础, 对非英语专业学生在不同的阅读任务下所采取的阅读策略的对比研究, 分析了不同学习成绩组学生与具体阅读任务下策略运用的相关数据, 了解学生在不同阅读任务下使用阅读策略的特点和变化, 以期更加有效地指导学生阅读、帮助教师更好地进行阅读教学。     

高职高专酒店管理专业学生实习中存在的问题及对策

从学生、校方以及酒店三个方面分析高职高专酒店管理专业学生实习中存在的问题,并提出对策。